The aim of this study was to isolate the probiotic bacteria from fermented food (Batter and Yakult) and check its impact on pathogenic biofilm. Two different selective media LB agar and LMRS agar were used. Samples were serially diluted and spread plate technique was executed to isolate the colonies. The colonies grown on the agar were characterized for various traits including Gram staining and morphology detection. Using the isolated intracellular products and pathogens (Staphylococcus aureus and Proteus mirabilis) antimicrobial assay was performed to check if the isolated lactobacillus were able to reduce the bacterial permission. Antibiofilm assay was performed by using the pathogenic microorganisms and lactobacillus. Altogether the study concludes that there are potential probiotics that can inhibit the growth of pathogenic biofilm