Antimicrobial resistance (AMR) is not a recent development, and it has become a major public health concern in recent decades. Bacteria that cause common infections have acquired resistance to each new antibiotic in varied degrees over several decades, and AMR has evolved into a global health issue. Amicoumacin A has potent antibacterial activity against methicillin-resistant Staphylococcus aureus (MRSA). A transcriptome-wide analysis revealed variations in the transcription of genes involved in a wide variety of cellular activities.DNA microarray analysis has emerged as a relatively new technique which is capable of detecting resistance genes in a short time as effectively as standard techniques and GEO2R is a tool which can validate its results by analyzing and studying the genes Insilco using R. Gene ontology was performed on the set of genes obtained from two tests at 10 and 40 minutes respectively and one control obtained from performing GEO2R analysis. The results obtained were used to do String analysis in two categories namely biosynthesis of antibiotics and secondary metabolites. This study shows the comparison between experimental and curated data in GEO2R analysis and discovers the genes responsible towards anti resistant properties of Amicoumacin A