The aim of this work was to identify the prevalence of CRISPR-Cas gene containing Klebsiella pneumoniae from contaminated water and soil. Out of 3 environmental samples 14 isolates were from Enterobacteriaceae family of which 2 were found to be K. pneumoniae. The identification of the isolates was done using various biochemical tests and 16S rRNA gene sequencing. With the use of disk diffusion method, the isolates showed resistance to certain antibiotics like Ciprofloxacin (CIP5), Cefazolin (CZ30) and Ampicillin/sulbactam (A/S). The isolate which was susceptible to all antibiotics tested showed the presence of CRISPR-Cas type I-E.